Tuesday, November 29, 2016

Osphronemus gouramy is a Freshwater Fish that Widely Cultivated in Indonesia



Seeds of carp (Osprhronemus goramy) strain Soang

Gourami (Osprhronemus goramy Lac.) is a freshwater fish that widely cultivated in Indonesia, particularly by fish farmer in Java. There are several strains of gourami, one of which is gourami soang strain. Soang strain has a faster growth rate than other strains. However, the fry that derived from a single cohort (generated from a single spawning) shows various sizes even in the same age due to has different growth rate. Differences in growth rate may occur due to differences in metabolic capabilities especially cellular respiration. Differences rate of respiration can be assumed because of differences in the genetic componen, especially on their cytochrome c oxidase 1 gene. Therefore, this study aimed to determine whether there are differences in PCR-RFLP marker cytochrome c oxidase 1 (CO1) gene in the gourami soang strain that have different sizes which coming from a cohort so that can be used to analyze genetic diversity. This study used survey method by applying purposive random sampling. Four restriction enzymes were used, molecular characters were defined descriptively based on the appearance of restriction fragment on agarose gel. The result showed that HindIII could cut the PCR product in all individuals, but PstI, BamHI, and EcoRI were not. This means that the gene does not show individual variation, or showed monomorphic on their CO1-HindIII. The three different sizes of soang strain has similar alleles or homozygous, so that heterozigocity value is 0 (zero). Because of that, cytochrome c oxidase 1 gene can not be used as molecular character to differentiate different sizes of gourami soang strain from the same cohort.


Key words : Gourami soang strain, PCR-RFLP, genetic diversity, cohort, cytochrome c oxidase 1 gene (CO1).




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